PCR Technique
PCR What Is PCR - PCR is Polymerase Chain Reaction used for making million copy of particular section of DNA . In 1983 Kary Mullis developed this technique used in variety of application like in medical case , criminal case , in diagnosis of genetic diseases and so on . For this work Mullis awarded by Noble prize in 1993 . PCR is rapid , inexpensive and simple way of copying of DNA fragments by using smaller quantity of source of DNA material PCR Tools - PCR can not be accomplish without this key tool - Template DNA Nucleotides ( dATP , dCTP , dGTP , dTTP ) Primer Taq Polymerase Enzyme Buffer for stability of DNA polymerase . Working Of PCR - PCR is a chain reaction as it synthesize DNA strands and act as template for further cycles . There are following stages to complete this process - Denaturation - In this stage the double stranded DNA is make single