Biology Blogs For Class11th, 12th and NEET

                                Human Cloning   Giving birth to the  identical copies of  the biological entity  by various clone process is known as cloning and when this  process used to produce identical copies of human is known as Human Cloning . But Human Cloning is very controversial in many aspects . This topic becomes the most important aspect of research for the scientist . How Human Cloning Work -  The most preferred method scientist use is somatic cell nuclear transfer in which the egg of the donor person  is taken out  and from this  egg nucleus is removed . Now this egg become unnucleated egg . This egg is ready for fusing with the cloning subject cell by using electricity . Cell which contain DNA is taken out from the person who is  being cloned . Once the two cell get fused  the new cell can be permitted to grow in an surrogate mother . The sheep - Dolly also produce by this method . Future Of Human Cloning - 

                                   Hybridoma Technology  Hybridoma Technology is the technology in which hybrid cells are produced by fusing a specific antibody producing B-cell with myeloma cell . The process start  by injecting a specific antigen  into a mouse and collect the antibodies producing cell from the spleen and fuse with the myeloma cells . Both of this fusing partner give hybrid cell known as Hybridoma cells . Now these cells are transferred to a medium in which the myeloma cells die , but the hybridomas survive . Therefore these cells are transferred  into HAT medium and aminopterin are also added . The work of aminopterin is to block de novo synthesis . Normally the cells are survive in this medium but if the cell are unable to  produce the HGPRT medium then they are unable to use de novo pathway and die in aminopterin containing medium .  After fusion of lymphocytes with HGPRT - negative myeloma  cells in an aminopterin containing medium which is supplemented wi