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Showing posts from July 17, 2016

Human Cloning : The Topic Of Debate

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                                Human Cloning   Giving birth to the  identical copies of  the biological entity  by various clone process is known as cloning and when this  process used to produce identical copies of human is known as Human Cloning . But Human Cloning is very controversial in many aspects . This topic becomes the most important aspect of research for the scientist . How Human Cloning Work -  The most preferred method scientist use is somatic cell nuclear transfer in which the egg of the donor person  is taken out  and from this  egg nucleus is removed . Now this egg become unnucleated egg . This egg is ready for fusing with the cloning subject cell by using electricity . Cell which contain DNA is taken out from the person who is  being cloned . Once the two cell get fused  the new cell can be permitted to grow in an su...

Hybridoma Technology: Process, Applications & Monoclonal Antibody Production

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                                   Hybridoma Technology  Hybridoma Technology is a method used to produce hybrid cells by fusing a specific antibody-producing B-cell with a myeloma (cancer) cell. The process begins by injecting a specific antigen into a mouse to stimulate an immune response. The antibody-producing B-cells are then collected from the mouse’s spleen and fused with myeloma cells. This fusion results in hybrid cells , known as hybridomas , which have two key properties: the ability to produce a specific antibody (from the B-cell) and the ability to divide indefinitely (from the myeloma cell). The fused cells are then placed in a special culture medium where the unfused myeloma cells die, but the hybridomas survive. This is achieved by using HAT medium (which contains hypoxanthine, aminopterin, and thymidine). Aminopterin in the medium blocks the de novo nucleotide synt...