Hybridoma Technology

-

                                   Hybridoma Technology 




Hybridoma Technology is the technology in which hybrid cells are produced by fusing a specific antibody producing B-cell with myeloma cell . The process start  by injecting a specific antigen  into a mouse and collect the antibodies producing cell from the spleen and fuse with the myeloma cells . Both of this fusing partner give hybrid cell known as Hybridoma cells . Now these cells are transferred to a medium in which the myeloma cells die , but the hybridomas survive . Therefore these cells are transferred  into HAT medium and aminopterin are also added . The work of aminopterin is to block de novo synthesis . Normally the cells are survive in this medium but if the cell are unable to  produce the HGPRT medium then they are unable to use de novo pathway and die in aminopterin containing medium .  After fusion of lymphocytes with HGPRT - negative myeloma  cells in an aminopterin containing medium which is supplemented with hypoxanthine and thymidine to ensure sufficient supply of substrate for the salvage pathway is added which kills myeloma cells but allow hybridomas to survive and in this way monoclonal antibodies are produced .

Comments

Post a Comment

Popular posts from this blog

Sycon Canal System With Diagram

-
Image
                                     Canal system in Sycon  A sea creature called a sycon, or scypha , is usually found clinging to rocks or other surfaces. These creatures have tube-shaped bodies that open outward with the help of a hole called an Osculum . They have tiny openings called Spiracles and Ostia all over their bodies to let water in. This water flows through canals inside their bodies and comes out through the Osculum, which is a larger opening used for breathing. We'll observe the shape of this canal system, why it is essential for those animals, type of canal system , and  its significance. The Sycon Type Canal system is the unique feature of the phylum Porifera . This system is also known as the  aquiferous canal system . This system supports the significant function of the body like nutrition, respiration, excretion, etc. History Overview -  Grant, a scientist from Scotland, spent a lot of time studying sponges. He was one of the first to really explain how t
Read more

How Many Types of Eggs In Animals

-
Image
                                             Types of Egg Hello guys! We'll examine a crucial area of zoology in this blog: different varieties of eggs. So let's define reproduction first before moving on to the subject. Reproduction is the biological process in which a parent is responsible to produce their offsprings which may be of their own kind. Reproduction is a must process for every individual for the continuing existence of the species. As we all know, there are many different species on the planet, and each of them has a unique method of egg production and inside the egg they contain yolk . The yolk is composed of proteins, fats, vitamins, and minerals, which are vital for the growth and development of the embryo.  Eggs can be classified into different types based on the amount of yolk they contain. l.  On the basis of the amount of Yolk - Depending on the quantity of yolks, eggs can be  Alecithal / Microlecithal / oligolecithal - The amount of yolk pr
Read more

Aurelia Aurita Life Cycle: Characterstics, Classification

-
Image
                                             Aurelia  Aurelia aurita is a type of zooplankton found all over the world. They are see-through and don't have eyes, brains, or hearts. They're usually between 2 to 15 inches wide. Even though they prefer being alone, they get carried together in groups called Blooms by wind and water currents. At night, they look like they're moving through the water like jellyfish, which is why they're sometimes called " jellyfish ".  They have four reproductive organs surrounded by tentacles in their top part. Their body color changes based on what they eat, so they can be blue, pink, or other colors. Let's learn more about Aurelia in detail. Details Of Aurelia Diagram -  Body : The center of the diagram would display the jellyfish's bell-shaped body, often called the " umbrella. " Tentacles : Below the bell, you'd see long, hair-like tentacles extending outward. Oral Arms : The oral arms are  used for
Read more