Electrophoresis
Southern Blotting In molecular biology there are three techniques used for detecting DNA / RNA /protein , Southern blotting for DNA detection , Northern blotting for RNA detection and Western blotting for detecting protein . The term blotting is generally refers to the transferring of the DNA /RNA / Protein into carrier like nylon membrane . After blotting the transferred molecules are visualized by autoradiography , chromogenic detection method , silver staining method etc . This technique was developed by Edwin Southern in 1975 . Procedure - The DNA is isolated from the cell of interest . Now the DNA is digested by restriction enzyme . Restriction enzyme cut the DNA molecules into desired fragments . The fragments are separated by agrose gel electrophoresis into different size . The double s...