Northern blot technique Of RNA - Principle , Steps | Biology Blog
Northern blotting Hello guys! In this blog, we are going to explore the northern blot technique . This technique can be used in any viral detection test as the genetic material of the virus is RNA. So, before moving to northern blot we focus on the word - blot. Blo t is basically a technique in molecular biology and genetics in which we DNA/RNA or proteins could be transferred onto the carrier membrane. The nylon membrane or nitrocellulose membrane are used for blotting purpose. The transferring of molecules like DNA / RNA or proteins to the membrane can take place after gel electrophoresis. After blotting the transferred molecules are visualized. For visualization, we can use the following methods- 1. If the probe is labeled with the radioactive chemical (32P), it can be observed on...